User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/09/17: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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Yesterday and today, we ran pH and conductivity calibrations for 50 mM NaCl and 50 mM Glycine buffers (pH 3.5, 0.4 mL 1M HCl used). A 2x, 5z, 10x, 20x, 50x, 100x, 200x, and 500x dilution of each was prepared for use. | Yesterday and today, we ran pH and conductivity calibrations for 50 mM NaCl and 50 mM Glycine buffers (pH 3.5, 0.4 mL 1M HCl used). A 2x, 5z, 10x, 20x, 50x, 100x, 200x, and 500x dilution of each was prepared for use. | ||
[[Image:NaCl_Glycine_Tables_17_Sept.png|500px]] | |||
The results are as follows: | The results are as follows: | ||
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'''NaCl''' | '''NaCl''' | ||
[[Image:NaCl_Conductivity_17_Sept.png]] | [[Image:NaCl_Conductivity_17_Sept.png|500px]] | ||
[[Image:NaCl_Log_Conc_pH_17_Sept.png]] | [[Image:NaCl_Log_Conc_pH_17_Sept.png|500px]] | ||
[[Image:NaCl_pH_17_Sept.png]] | [[Image:NaCl_pH_17_Sept.png|500px]] | ||
'''Glycine''' | '''Glycine''' | ||
[[Image:Glycine_Conductivity_17_Sept.png]] | [[Image:Glycine_Conductivity_17_Sept.png|500px]] | ||
[[Image:Glycine_Log_Conc_pH_17_Sept.png|500px]] | |||
[[Image:Glycine_pH_17_Sept.png|500px]] | |||
==Lysozyme 1g/L Data== | |||
pH and conductivity were taken for the stock solution and for a 1 in 6 dilution. | |||
#Stock | |||
##pH - 5.700 | |||
##Conductivity - 144.6 µS/cm | |||
#1 in 6 Dilution | |||
##pH - 6.123 | |||
##Conductivity - 24.14 µS/cm | |||
==Dialysis== | ==Dialysis== | ||
Latest revision as of 00:18, 27 September 2017
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pH and Conductivity Calibration of NaCl and Glycine BuffersYesterday and today, we ran pH and conductivity calibrations for 50 mM NaCl and 50 mM Glycine buffers (pH 3.5, 0.4 mL 1M HCl used). A 2x, 5z, 10x, 20x, 50x, 100x, 200x, and 500x dilution of each was prepared for use.
The results are as follows: NaCl
Glycine
Lysozyme 1g/L DatapH and conductivity were taken for the stock solution and for a 1 in 6 dilution.
DialysisWe used the two buffers to run dialysis of 1 g/L lysozyme. We used 3500 and 25k MW dialysis tubing. The 3500 was used for NaCl, Glycine, and H2O, and colloid solution (made on 26 August). These were left overnight to today.
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