User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/10/01: Difference between revisions
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Bradford analysis of the dialysis was run for the soak solution and the protein solution. The results are below, along with calculated concentrations: | Bradford analysis of the dialysis was run for the soak solution and the protein solution. The results are below, along with calculated concentrations: | ||
[[Image: | [[Image:Bradford_Dialysis_10_1.png|500px]] | ||
The soak solutions were not included in this graph, because the MW cutoff of the dialysis membrane prevented the Lysozyme from moving across the membrane. | |||
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A second dialysis was prepared using 2 wells of 0.12 g/L lysozyme and the 30:1 Au:Lysozyme colloid against 20k MW paper. The dialysis will run for a week. | A second dialysis was prepared using 2 wells of 0.12 g/L lysozyme and the 30:1 Au:Lysozyme colloid against 20k MW paper. These were run against 50 mM, 500 µM, and 5 µM CaCl<sub>2</sub>. The dialysis will run for a week. | ||
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Bradford Analysis of Lysozyme DialysisBradford analysis of the dialysis was run for the soak solution and the protein solution. The results are below, along with calculated concentrations: The soak solutions were not included in this graph, because the MW cutoff of the dialysis membrane prevented the Lysozyme from moving across the membrane.
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