User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/02/18: Difference between revisions
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== | ==10 nM Protease K Kinetics Run== | ||
'''Objective''' | |||
Determine the kinetics of Protease K on AuNP fibers at 10 nM enzyme concentration | |||
'''Protocol''' | |||
69 μL of the 0.4325 μM stock was diluted with 2.931 mL of Tris/NaCl buffer. This was added onto the fibers in the cuvette. The reaction was run at 37°C for 6 hours, scanning every 5 minutes. | |||
'''Results''' | |||
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10 nM Protease K Kinetics RunObjective Determine the kinetics of Protease K on AuNP fibers at 10 nM enzyme concentration Protocol 69 μL of the 0.4325 μM stock was diluted with 2.931 mL of Tris/NaCl buffer. This was added onto the fibers in the cuvette. The reaction was run at 37°C for 6 hours, scanning every 5 minutes. Results
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