User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/03/03: Difference between revisions
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'''Results''' | '''Results''' | ||
[[Image:1nM_Trypsin_AbsvsTime_Chart.png|500px]] | |||
[[Image:1nM_Trypsin_Kinetics_Chart.png|500px]] | |||
There was barely a change in absorbance with this concentration of Trypsin. | |||
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1 nM Trypsin In Situ KineticsRan the final Trypsin In Situ reaction. Protocol 9.07 µL of the diluted stock was added to 2.991 mL of Tris/NaCl buffer. Fibers were spun at 300 RPM for 20 minutes (the fibers did not sediment after the first 10 minutes), and the liquid was extracted. 2 mL of the Trypsin was added to the cuvette with minimum amount of liquid from AuNP fibers. OceanOptics was run at 37°C, spinning at 1000 RPM, scanning every 5 minutes for 6 hours total. Results
There was barely a change in absorbance with this concentration of Trypsin. | |
