User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/03/04: Difference between revisions

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'''Results'''
'''Results'''
[[Image:1uM_Chymotrypsin_Absvstime_Mar_4_Chart.png|500px]]
[[Image:1uM_Chymotrypsin_Kinetics_Mar_4_Chart.png|500px]]





Revision as of 14:49, 31 March 2015

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1 μM Chymotrypsin Kinetics

Protocol

Switching to Chymotrypsin kinetics. Same protocol for OceanOptics, scan every 30 seconds for 2 hours at 37°C, spinning at 1000 RPM.

For the solution prep, 104 μL of concentrated Chymotrypsin stock (24.00 μM) was diluted by 2.896 mL of Tris-10mM CaCl2 buffer.

Results