User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/03/17: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==100 nM Chymotrypsin In Situ Kinetics== | ==100 nM Chymotrypsin In Situ Kinetics== | ||
10.4 μL of the stock was diluted with 1.99 mL Tris/NaCl/CaCl<sub>2</sub> buffer. | |||
[[Image:100nM_Chymotrypsin_AbsvsTime_Mar17_Chart.png|500px]] | |||
[[Image:100nM_Chymotrypsin_Kinetics_Mar17_Chart.png|500px]] | |||
=='''Bradford Analysis of Thermolysin Reaction Samples'''== | =='''Bradford Analysis of Thermolysin Reaction Samples'''== | ||
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250 μL of each protein sample at every time point was combined with 200 μL of Bradford reagent (1:4 Bradford:Tris/CaCl<sub>2</sub>), and 550 mL of Tris/CaCl<sub>2</sub> buffer and run from 400 - 800 nm on the UV/Vis spectrophotometer. The following are the results with the blank subtracted out and zeroed at a wavelength of 535 nm. | 250 μL of each protein sample at every time point was combined with 200 μL of Bradford reagent (1:4 Bradford:Tris/CaCl<sub>2</sub>), and 550 mL of Tris/CaCl<sub>2</sub> buffer and run from 400 - 800 nm on the UV/Vis spectrophotometer. The following are the results with the blank subtracted out and zeroed at a wavelength of 535 nm. | ||
[[Image:Bradford_kinetics_1uM_thermolysin.png]] | [[Image:Bradford_kinetics_1uM_thermolysin.png|500px]] | ||
[[Image:Bradford_kinetics_100nM_thermolysin.png]] | [[Image:Bradford_kinetics_100nM_thermolysin.png|500px]] | ||
[[Image:Bradford_kinetics_10nM_thermolysin.png]] | [[Image:Bradford_kinetics_10nM_thermolysin.png|500px]] | ||
[[Image:Bradford_kinetics_1nM_thermolysin.png]] | [[Image:Bradford_kinetics_1nM_thermolysin.png|500px]] | ||
Latest revision as of 00:49, 27 September 2017
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100 nM Chymotrypsin In Situ Kinetics10.4 μL of the stock was diluted with 1.99 mL Tris/NaCl/CaCl2 buffer.
Bradford Analysis of Thermolysin Reaction Samples250 μL of each protein sample at every time point was combined with 200 μL of Bradford reagent (1:4 Bradford:Tris/CaCl2), and 550 mL of Tris/CaCl2 buffer and run from 400 - 800 nm on the UV/Vis spectrophotometer. The following are the results with the blank subtracted out and zeroed at a wavelength of 535 nm.
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