User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/03/17: Difference between revisions
From OpenWetWare
| Line 8: | Line 8: | ||
==100 nM Chymotrypsin In Situ Kinetics== | ==100 nM Chymotrypsin In Situ Kinetics== | ||
10.4 μL of the stock was diluted with 1.99 mL Tris/NaCl/CaCl<sub>2</sub> buffer. | |||
[[Image:100nM_Chymotrypsin_AbsvsTime_Mar17_Chart.png|500px]] | |||
[[Image:100nM_Chymotrypsin_Kinetics_Mar17_Chart.png|500px]] | |||
=='''Bradford Analysis of Thermolysin Reaction Samples'''== | =='''Bradford Analysis of Thermolysin Reaction Samples'''== | ||
Revision as of 14:47, 31 March 2015
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
100 nM Chymotrypsin In Situ Kinetics10.4 μL of the stock was diluted with 1.99 mL Tris/NaCl/CaCl2 buffer.
Bradford Analysis of Thermolysin Reaction Samples250 μL of each protein sample at every time point was combined with 200 μL of Bradford reagent (1:4 Bradford:Tris/CaCl2), and 550 mL of Tris/CaCl2 buffer and run from 400 - 800 nm on the UV/Vis spectrophotometer. The following are the results with the blank subtracted out and zeroed at a wavelength of 535 nm.
| |
