User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/03/31: Difference between revisions
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#Prepare AuNP fibers - vortex each 5 mL test tube containing fibers and combine some necessary amount into a large falcon tube. | #Prepare AuNP fibers - vortex each 5 mL test tube containing fibers and combine some necessary amount into a large falcon tube. | ||
#Centrifuge the falcon tube allowing the fibers to settle at the bottom and pour off the supernatant liquid. | #Centrifuge the falcon tube allowing the fibers to settle at the bottom and pour off the supernatant liquid. | ||
#Add Tris/CaCl<sub>2</sub> buffer and vortex again, resulting in a homogenous solution of fibers which can be equipartitioned between 20 sampling epi-tubes. | #Add Tris/CaCl<sub>2</sub> buffer and vortex again, resulting in a homogenous solution of fibers which can be equipartitioned between 20 sampling epi-tubes. | ||
#Add protease to each epi tube. (1 epi-tube per each timepoint being sampled) | #Add protease to each epi tube. (1 epi-tube per each timepoint being sampled) | ||
#At 0.5, 1, 1.5, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 40, 50, 60 min stop the reaction by removing from the "incubator/shaker" and centrifuging for 30 sec. | #At 0.5, 1, 1.5, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 40, 50, 60 min stop the reaction by removing from the "incubator/shaker" and centrifuging for 30 sec. | ||
#Collect the supernatant for analysis. | #Collect the supernatant for analysis. | ||
Here's the new protocol we developed for analysis. | Here's the new protocol we developed for analysis. | ||
==Notes from Protocol Prep== | ==Notes from Protocol Prep== |
Revision as of 09:34, 31 March 2015
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New Reaction/Sampling Protocol
Here's the new protocol we developed for analysis. Notes from Protocol Prep
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