User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/04/07: Difference between revisions

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The protocol for SDS-PAGE was found [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24 here]. The BioRad protocol can be found [http://ergo.berkeley.edu/be115/protean3.pdf here].  
The protocol for SDS-PAGE was found [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/24 here]. The BioRad protocol can be found [http://ergo.berkeley.edu/be115/protean3.pdf here].  
'''Protocol:'''
#10 µL of the original sample was diluted to 1 mL with Gly-HCl buffer
#10 µL of the diluted sample was diluted with 10 µL of SDS-PAGE running buffer




Revision as of 11:52, 7 April 2015

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SDS-PAGE Preparation

Solutions were prepared for SDS-PAGE analysis (to be run tomorrow).

Solutions:

  1. 50 mM Glycine-HCl, pH 3
  2. Running buffer (prepared previously)

The protocol for SDS-PAGE was found here. The BioRad protocol can be found here.

Protocol:

  1. 10 µL of the original sample was diluted to 1 mL with Gly-HCl buffer
  2. 10 µL of the diluted sample was diluted with 10 µL of SDS-PAGE running buffer




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