User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/04/14: Difference between revisions

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We prepared non-dilute samples for SDS-PAGE analysis. Here are the solutions we used for each enzyme concentration:
We prepared non-dilute samples for SDS-PAGE analysis. Here are the solutions we used for each enzyme concentration:


#Proteinase K:
#<u>Proteinase K Sample 1</u>:
##1 µM, modified protocol, 40 min sample
##1 µM, 2 hrs
##100 nM, 2.5 hrs
##10 nM, 2 hrs
##1 nM, 2 hrs
#<u>Proteinase K Sample 2</u>:
##1 µM, modified protocol, 60 min  
##100 nM, modified, 80 min
##10 nM, modified, 22 hrs
##1 nM, modified, 21 hrs
#<u>Thermolysin</u>:
#<u>Thermolysin</u>:
##1 µM, 90 minute sample
##1 µM, 90 min
##100 nM, 30 minute sample
##100 nM, 30 min
##10 nM, 60 minute sample
##10 nM, 60 min
##1 nM, 10 minute sample
##1 nM, 10 min
 
#<Chymotrypsin</u>:
##1 µM, 1 hr
##100 nM, 25 min
##10 nM, 20 hrs
##1 nM, 23 hrs
#<u>Trypsin</u>:
##1 µM, 2 hrs
##100 nM, 2.5 hrs
##10 nM, 22 hrs
##1 nM, 21 hrs




Revision as of 12:45, 14 April 2015

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SDS-PAGE Sample Prep

We prepared non-dilute samples for SDS-PAGE analysis. Here are the solutions we used for each enzyme concentration:

  1. Proteinase K Sample 1:
    1. 1 µM, 2 hrs
    2. 100 nM, 2.5 hrs
    3. 10 nM, 2 hrs
    4. 1 nM, 2 hrs
  2. Proteinase K Sample 2:
    1. 1 µM, modified protocol, 60 min
    2. 100 nM, modified, 80 min
    3. 10 nM, modified, 22 hrs
    4. 1 nM, modified, 21 hrs
  3. Thermolysin:
    1. 1 µM, 90 min
    2. 100 nM, 30 min
    3. 10 nM, 60 min
    4. 1 nM, 10 min
  4. <Chymotrypsin:
    1. 1 µM, 1 hr
    2. 100 nM, 25 min
    3. 10 nM, 20 hrs
    4. 1 nM, 23 hrs
  5. Trypsin:
    1. 1 µM, 2 hrs
    2. 100 nM, 2.5 hrs
    3. 10 nM, 22 hrs
    4. 1 nM, 21 hrs



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