User:Paul Rothenberg/Notebook/Pauls Notebook/2012/02/22 - Revision history

Paul Rothenberg: /* 22 February 2012 */

Paul Rothenberg — Wed, 22 Feb 2012 21:41:19 GMT

22 February 2012

←Older revision		Revision as of 21:41, 22 February 2012
Line 7:		Line 7:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==22 February 2012==		==22 February 2012==
-	* Today, the proteins were isolated with a purification machine (GE AKTApurifier). The proteins were run through at 30% B solution (25mM Tris, 1M NaCl) in solution A (25 mM Tris, 50mM NaCl) to prevent the positively charged filter from attracting the negatively charged hemoglobin proteins.	+	* Today, the proteins were isolated with a purification machine (GE AKTApurifier). The proteins were run through at 30% B solution (25mM Tris, 1M NaCl) in solution A (25 mM Tris, 50mM NaCl) to prevent the positively charged filter from attracting the negatively charged hemoglobin proteins. After being purified, they were put into containers and refrigerated.

	* For February 15, the two solutions were mixed in the laboratory. Hemoglobin proteins without Heme were also sonicated to retroactively add Heme. This was done to three containers of hemoglobin. Each container was sonicated 2, 4, or 6 times, respectively.		* For February 15, the two solutions were mixed in the laboratory. Hemoglobin proteins without Heme were also sonicated to retroactively add Heme. This was done to three containers of hemoglobin. Each container was sonicated 2, 4, or 6 times, respectively.

Paul Rothenberg: /* Entry title */

Paul Rothenberg — Wed, 22 Feb 2012 19:51:58 GMT

Entry title

←Older revision		Revision as of 19:51, 22 February 2012
Line 6:		Line 6:
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==22 February 2012==
-	* ~~Insert content here~~...	+	* Today, the proteins were isolated with a purification machine (GE AKTApurifier). The proteins were run through at 30% B solution (25mM Tris, 1M NaCl) in solution A (25 mM Tris, 50mM NaCl) to prevent the positively charged filter from attracting the negatively charged hemoglobin proteins.
		+
		+	* For February 15, the two solutions were mixed in the laboratory. Hemoglobin proteins without Heme were also sonicated to retroactively add Heme. This was done to three containers of hemoglobin. Each container was sonicated 2, 4, or 6 times, respectively.

Paul Rothenberg: Autocreate 2012/02/22 Entry for User:Paul_Rothenberg/Notebook/Pauls_Notebook

Paul Rothenberg — Wed, 22 Feb 2012 19:45:46 GMT

Autocreate 2012/02/22 Entry for User:Paul_Rothenberg/Notebook/Pauls_Notebook

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__