User:Paul Rothenberg/Notebook/Pauls Notebook/2012/09/19

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*Procedure for 19 September 2012':
*Procedure for 19 September 2012':
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1. Amounts of Nickelous Sulfate and Protoporphyrin IX were measured out based on Stoichiometry (1).  
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*1. Amounts of Nickelous Sulfate and Protoporphyrin IX were measured out based on Stoichiometry (1).  
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2. 46 mg (44.2 mg) of Nickelous Sulfate and 100 mg (100.5 mg) Protoporphyrin IX were measured out on a balance.  
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*2. 46 mg (44.2 mg) of Nickelous Sulfate and 100 mg (100.5 mg) Protoporphyrin IX were measured out on a balance.  
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3. 10 mL DMF is added to a 50 mL round-bottom flask with a 19/22 Opening (1)
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*3. 10 mL DMF is added to a 50 mL round-bottom flask with a 19/22 Opening (1)
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4. The flask was placed on a heating pad with a 100 Volt Volt-ac with a stir bar under the presence of Nitrogen.  
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*4. The flask was placed on a heating pad with a 100 Volt Volt-ac with a stir bar under the presence of Nitrogen.  
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5. The DMF was brought to reflux under these conditions (1).
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*5. The DMF was brought to reflux under these conditions (1).
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6. Once the DMF was in reflux, the protoporphyrin IX was added, and let reflux (1).
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*6. Once the DMF was in reflux, the protoporphyrin IX was added, and let reflux (1).
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7. The Nickelous Sulfate was then added, and let reflux for 30 minutes (1).  
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*7. The Nickelous Sulfate was then added, and let reflux for 30 minutes (1).  
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8. The flask was then chilled in an ice bath for 15 minutes (1).  
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*8. The flask was then chilled in an ice bath for 15 minutes (1).  
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9. 100 mL of chilled water was then added, was left to chill for 10 minutes, and the precipitate is filtered using a Buchner funnel (1).  
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*9. 100 mL of chilled water was then added, was left to chill for 10 minutes, and the precipitate is filtered using a Buchner funnel (1).  
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10. The filtered material was washed with water (1).  
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*10. The filtered material was washed with water (1).  
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*See Dr. Harting's notebook for the filtration results.  
*See Dr. Harting's notebook for the filtration results.  

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19 September 2012

  • Procedure for 19 September 2012':
  • 1. Amounts of Nickelous Sulfate and Protoporphyrin IX were measured out based on Stoichiometry (1).
  • 2. 46 mg (44.2 mg) of Nickelous Sulfate and 100 mg (100.5 mg) Protoporphyrin IX were measured out on a balance.
  • 3. 10 mL DMF is added to a 50 mL round-bottom flask with a 19/22 Opening (1)
  • 4. The flask was placed on a heating pad with a 100 Volt Volt-ac with a stir bar under the presence of Nitrogen.
  • 5. The DMF was brought to reflux under these conditions (1).
  • 6. Once the DMF was in reflux, the protoporphyrin IX was added, and let reflux (1).
  • 7. The Nickelous Sulfate was then added, and let reflux for 30 minutes (1).
  • 8. The flask was then chilled in an ice bath for 15 minutes (1).
  • 9. 100 mL of chilled water was then added, was left to chill for 10 minutes, and the precipitate is filtered using a Buchner funnel (1).
  • 10. The filtered material was washed with water (1).



  • See Dr. Harting's notebook for the filtration results.


  • References:

1. Adler, Alan D. On the preparation of metalloporphyrins. Journal of Inorganic Nuclear Chemistry, Vol.32. 1970.



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