User:Paul Rothenberg/Notebook/Pauls Notebook/2013/02/04: Difference between revisions
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== | ==4 February 2013== | ||
* Insert | * Objectives: | ||
*1. Run UV-VIS and Fluorescence on protein dissolved in Chloroform in Acetate buffer and water in acetate buffer (See Dhea Patel's Notebook - Insert link) | |||
*2. Use a Lyopholizer to solidify proteins dissolved in solution | |||
*Lyopholizer Procedure: | |||
*1. The Lyopholizer was turned on and let cool to -50 Centigrade. | |||
*2. The proteins were solidified in liquid nitrogen, and placed in the vacuum | |||
*Fluorescence and UV-VIS Procedure: | |||
*1. Chloroform and water samples were run in each spectroscope | |||
2* | |||
*10 mg of Sorbitol, Hemoglobin, and PEG previously solidifed was added to 5 out of 6 tubes for solvent to be added (See Dhea's Notebook) | |||
*Data: | |||
* | |||
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