User:Paul Rothenberg/Notebook/Pauls Notebook/2013/02/04
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| - | == | + | ==4 February 2013== |
| - | * Insert | + | * Objectives: |
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| + | *1. Run UV-VIS and Fluorescence on protein dissolved in Chloroform in Acetate buffer and water in acetate buffer (See Dhea Patel's Notebook - Insert link) | ||
| + | *2. Use a Lyopholizer to solidify proteins dissolved in solution | ||
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| + | *Lyopholizer Procedure: | ||
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| + | *1. The Lyopholizer was turned on and let cool to -50 Centigrade. | ||
| + | *2. The proteins were solidified in liquid nitrogen, and placed in the vacuum | ||
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| + | *Fluorescence and UV-VIS Procedure: | ||
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| + | *1. Chloroform and water samples were run in each spectroscope | ||
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| + | *No data received from this procedure | ||
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| + | *10 mg of Sorbitol, Hemoglobin, and PEG previously solidifed was added to 5 out of 6 tubes for solvent to be added (See Dhea's Notebook) | ||
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| + | *Data: | ||
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| + | * | ||
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4 February 2013
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