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4 February 2013
- 1. Run UV-VIS and Fluorescence on protein dissolved in Chloroform in Acetate buffer and water in acetate buffer (See Dhea Patel's Notebook - Insert link)
- 2. Use a Lyopholizer to solidify proteins dissolved in solution
- 1. The Lyopholizer was turned on and let cool to -50 Centigrade.
- 2. The proteins were solidified in liquid nitrogen, and placed in the vacuum
- Fluorescence and UV-VIS Procedure:
- 1. Chloroform and water samples were run in each spectroscope
2*
- 10 mg of Sorbitol, Hemoglobin, and PEG previously solidifed was added to 5 out of 6 tubes for solvent to be added (See Dhea's Notebook)
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