User:Paul Rothenberg/Notebook/Pauls Notebook/2013/02/25: Difference between revisions
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== | ==25 February 2013== | ||
* | * Objectives: | ||
*1. Create a 1% agarose gel (1g agarose in 100 mL TAE) for gel electrophoresis | |||
*Data: | |||
*1. 50x TAE stock buffer was diluted to 1x TAE buffer, using 2 mL of 50x buffer and 98 mL of ultra-pure water (1) | |||
*2. 0.25 grams of agarose was dissolved in 25 mL of TAE | |||
*3. The TAE was microwaved for 45 seconds to dissolve the agarose | |||
*4. The TAE and agarose was poured into the electrophoresis chamber and left to harden; electrophoresis buffer was added following | |||
*5. 5 microliters of DNA and 1 microliter of dye; 6 microliters of the DNA ladder were added to the wells | |||
*References: | |||
*1.http://openwetware.org/wiki/1X_TAE | |||
Revision as of 10:08, 25 February 2013
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25 February 2013
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