User:Paul Rothenberg/Notebook/Pauls Notebook/2013/02/25: Difference between revisions
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*1. Create a 1% agarose gel (1g agarose in 100 mL TAE) for gel electrophoresis | *1. Create a 1% agarose gel (1g agarose in 100 mL TAE) for gel electrophoresis | ||
*2. Run a DNA sample to test for expression | |||
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*4. The TAE and agarose was poured into the electrophoresis chamber and left to harden; electrophoresis buffer was added following | *4. The TAE and agarose was poured into the electrophoresis chamber and left to harden; electrophoresis buffer was added following | ||
*5. 5 microliters of DNA and 1 microliter of dye; 6 microliters of the DNA ladder were added to the wells | *5. 5 microliters of DNA and 1 microliter of dye; 6 microliters of the DNA ladder were added to the wells | ||
*6. The gel was run for minutes at 90V | |||
Revision as of 10:58, 25 February 2013
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25 February 2013
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