User:Paul Rothenberg/Notebook/Pauls Notebook/2013/03/04

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(4 March 2013)
Current revision (13:48, 4 March 2013) (view source)
(4 March 2013)
 

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4 March 2013

  • Objectives:
  • 1. Synthesize two buffers to purify Nickel Hemoglobin previously synthesized
  • Procedure:
  • 1. 10 mM Tris (1.21g) and 50 mM NaCl (2.922g) were added, along with 900 mL of distilled water
  • 2. The pH was brought to 9.5 using 1M NaOH and 3 M HCl, and the remaining 100 mL of distilled water were added


  • 3. 10 mM Tris (1.21g) and 1M NaCl (58.44g) were added with 900 mL of distilled water
  • 4. The pH was brought to 9.5 using 1M NaOH, and the remaining 100 mL of distilled water were added


  • 5. 10 mM Tris and 50 mM NaCl were added along with 3,975 mL of distilled water
  • 6. The pH was brought to 9.5 using 3M HCl, and the remaining 25 mL of water were added
  • 7. The resulting buffer was used as a dialysis solution, exchanging previously synthesized Ni Hb in pH 8.5 solution
  • 8. The Ni Hb was placed in two dialysis bags and placed in the pH 9.5 solution in a cold environment, left overnight



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