User:Paul Rothenberg/Notebook/Pauls Notebook/2013/03/18

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18 March 2013 Objectives: 1. Run UV-VIS and Fluorescence spectroscopy on prepared solutions of proteins in DMSO Procedure: 1. A control sample of 200 µL DMSO was run in UV-VIS and Fluorescence spectroscopes 2. 200 µL of Mb, DMSO, Crown, and Phosphate solution was run 3. 190 µL of DMSO and 10 µL of Mb, DMSO, Crown, Phosphate was run 4. 199 µL of DMSO and 1 µL of Mb, DMSO, Crown, Phosphate was run 5. 25 µL of [4] was diluted with 175 µL of DMSO 6. 200 µL of Mb, Acetate, DMSO, Crown was run 7. 20 µL of [6] was diluted with 180 µL DMSO (10x factor) 8. 20 µL of [7] was diluted with 180 µL DMSO (10x factor) Data: 1. The control spectra showed signs of contamination in the cuvettes; aquaregia was used to clean the cuvettes 2. DMSO showed a peak between 200-300 nm in UV-VIS spectrum and a large peak in Fluorescence spectroscopy 3. The 200 µL of DMSO, Crown, Phosphate was too concentrated − diluted 4. [3] was still too concentrated under fluorescence 5. [4] was too concentrated − removed and saved; 25 µL of [4] was added along with 175 µL DMSO 6. Concentration of [5] did not max out the fluorimeter 7. [6] was highly concentrated under UV-VIS, and not run under Fluorescence 8. [7] was too concentrated under fluorescence, and diluted 9. [8] did not max out the fluorimeter or the UV-VIS