User:Paul Rothenberg/Notebook/Pauls Notebook/2013/03/18: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/03/18 Entry for User:Paul_Rothenberg/Notebook/Pauls_Notebook)
 
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
=18 March 2013==
* Insert content here...
* Objectives:
 
*1. Run UV-VIS and Fluorescence spectroscopy on prepared solutions of proteins in DMSO
 
*Procedure:
 
*1. A control sample of 200 µL DMSO was run in UV-VIS and Fluorescence spectroscopes
*2. 200 µL of Mb, DMSO, Crown, and Phosphate solution was run
*3. 190 µL of DMSO and 10 µL of Mb, DMSO, Crown, Phosphate was run
*4. 199 µL of DMSO and 1 µL of Mb, DMSO, Crown, Phosphate was run
*5. 25 µL of [4] was diluted with 175 µL of DMSO

*6. 200 µL of Mb, Acetate, DMSO, Crown was run
*7. 20 µL of [6] was diluted with 180 µL DMSO (10x factor)
*8. 20 µL of [7] was diluted with 180 µL DMSO (10x factor)
 
*Data:
 
*1. The control spectra showed signs of contamination in the cuvettes; aquaregia was used to clean the cuvettes
*2. DMSO showed a peak between 200-300 nm in UV-VIS spectrum and a large peak in Fluorescence spectroscopy
*3. The 200 µL of DMSO, Crown, Phosphate was too concentrated − diluted
*4. [3] was still too concentrated under fluorescence
*5. [4] was too concentrated − removed and saved; 25 µL of [4] was added along with 175 µL DMSO
*6. Concentration of [5] did not max out the fluorimeter

*7. [6] was highly concentrated under UV-VIS, and not run under Fluorescence
*8. [7] was too concentrated under fluorescence, and diluted
*9. [8] did not max out the fluorimeter or the UV-VIS




Revision as of 12:11, 18 March 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

18 March 2013=

  • Objectives:
  • 1. Run UV-VIS and Fluorescence spectroscopy on prepared solutions of proteins in DMSO
  • Procedure:
  • 1. A control sample of 200 µL DMSO was run in UV-VIS and Fluorescence spectroscopes
  • 2. 200 µL of Mb, DMSO, Crown, and Phosphate solution was run
  • 3. 190 µL of DMSO and 10 µL of Mb, DMSO, Crown, Phosphate was run
  • 4. 199 µL of DMSO and 1 µL of Mb, DMSO, Crown, Phosphate was run
  • 5. 25 µL of [4] was diluted with 175 µL of DMSO

  • 6. 200 µL of Mb, Acetate, DMSO, Crown was run
  • 7. 20 µL of [6] was diluted with 180 µL DMSO (10x factor)
  • 8. 20 µL of [7] was diluted with 180 µL DMSO (10x factor)
  • Data:
  • 1. The control spectra showed signs of contamination in the cuvettes; aquaregia was used to clean the cuvettes
  • 2. DMSO showed a peak between 200-300 nm in UV-VIS spectrum and a large peak in Fluorescence spectroscopy
  • 3. The 200 µL of DMSO, Crown, Phosphate was too concentrated − diluted
  • 4. [3] was still too concentrated under fluorescence
  • 5. [4] was too concentrated − removed and saved; 25 µL of [4] was added along with 175 µL DMSO
  • 6. Concentration of [5] did not max out the fluorimeter

  • 7. [6] was highly concentrated under UV-VIS, and not run under Fluorescence
  • 8. [7] was too concentrated under fluorescence, and diluted
  • 9. [8] did not max out the fluorimeter or the UV-VIS



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/03/18&oldid=683782"