The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
|
18 March 2013=
- 1. Run UV-VIS and Fluorescence spectroscopy on prepared solutions of proteins in DMSO
- 1. A control sample of 200 µL DMSO was run in UV-VIS and Fluorescence spectroscopes
- 2. 200 µL of Mb, DMSO, Crown, and Phosphate solution was run
- 3. 190 µL of DMSO and 10 µL of Mb, DMSO, Crown, Phosphate was run
- 4. 199 µL of DMSO and 1 µL of Mb, DMSO, Crown, Phosphate was run
- 5. 25 µL of [4] was diluted with 175 µL of DMSO
- 6. 200 µL of Mb, Acetate, DMSO, Crown was run
- 7. 20 µL of [6] was diluted with 180 µL DMSO (10x factor)
- 8. 20 µL of [7] was diluted with 180 µL DMSO (10x factor)
- 1. The control spectra showed signs of contamination in the cuvettes; aquaregia was used to clean the cuvettes
- 2. DMSO showed a peak between 200-300 nm in UV-VIS spectrum and a large peak in Fluorescence spectroscopy
- 3. The 200 µL of DMSO, Crown, Phosphate was too concentrated − diluted
- 4. [3] was still too concentrated under fluorescence
- 5. [4] was too concentrated − removed and saved; 25 µL of [4] was added along with 175 µL DMSO
- 6. Concentration of [5] did not max out the fluorimeter
- 7. [6] was highly concentrated under UV-VIS, and not run under Fluorescence
- 8. [7] was too concentrated under fluorescence, and diluted
- 9. [8] did not max out the fluorimeter or the UV-VIS
|
Project name
