User:Paul Rothenberg/Notebook/Pauls Notebook/2013/05/22: Difference between revisions

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*2. 20 mL of each buffer was added (pH 9 Tris; pH 7 Phosphate; pH 5 Acetate; pH 3 Citrate)
*2. 20 mL of each buffer was added (pH 9 Tris; pH 7 Phosphate; pH 5 Acetate; pH 3 Citrate)
*3. The solutions were frozen in liquid nitrogen and lyopholized
*3. The solutions were frozen in liquid nitrogen and lyopholized
*Graphs:
[[Image:5C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png]]
[[Image:15C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png]]
[[Image:25C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png]]
[[Image:35C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png]]
[[Image:45C_Mb_Phosphate_OPD_H2O2_MeOH_WORKUP_GRAPH.png]]




Revision as of 12:44, 24 May 2013

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22 May 2013

  • Objectives:
  • 1. Continue analysis of reaction between OPD and Hydrogen Peroxide with Mb catalyst in Phosphate buffer
  • 2. Lyopholize more solutions of Mb in buffer for reaction
  • Procedure:
  • 1. Same as [5/20/13 and 5/21/13], except with Phosphate buffer


  • Lyopholization of Mb in Buffers:
  • 1. 5 milligrams of Myoglobin and 490 milligrams of KCl were added to four tubes
  • 2. 20 mL of each buffer was added (pH 9 Tris; pH 7 Phosphate; pH 5 Acetate; pH 3 Citrate)
  • 3. The solutions were frozen in liquid nitrogen and lyopholized
  • Graphs:



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