User:Paul Rothenberg/Notebook/Pauls Notebook/2013/05/31

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Reaction of OPD+H2O2 with Mb/Citrate Catalyst in Suspension of Isopropanol

Going to try something different: instead of dissolving everything in solution, I am only going to suspend it in Isopropanol. The stock solutions will be made in the same volumes (only with Isopropanol), but the reaction will not be run 5 times. Instead, the reactants will total approximately 10 mL and will be sonicated before the H2O2 will be added.

For this reaction, 0.75 mL of the Mb/Citrate solution, 6.74 mL of OPD, and 2.51 mL of H2O2 will be reacted, for a total of 10 mL of liquid in the tube.

The reaction was started at 11:05. The first aliquot will be removed at 12:05 and a spectra will be taken. This will be done every hour.

12:05: The reaction is definitely proceeding forwards, but the tube must be shaken to prevent any reactants from falling out of solution. The solvent is definitely changing color, indicating the presence of the reaction.

1:05: The reaction was much darker than 1 hour ago, and the absorbances show that. Still needs to be shaken to keep everything suspended from falling out. Will update more when graphs are made.

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