User:Paul Rothenberg/Notebook/Pauls Notebook/2013/07/01: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Reaction of OPD+H<sub>2</sub>O<sub>2</sub> with Mb/Acetate Catalyst in H<sub>2</sub>O== | ||
Going to continue the trials in H<sub>2</sub>O with Mb/Acetate. We are also going to make up more buffered protein solutions. | |||
<b>Trial 2 Data:</b> | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_5C_GRAPH_Trial2.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_5C_SEQUENTIAL_GRAPH_Trial2.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_15C_GRAPH_Trial2.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_15C_SEQUENTIAL_GRAPH_Trial2.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_25C_GRAPH_Trial2.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_25C_SEQUENTIAL_GRAPH_Trial2.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_35C_GRAPH_Trial2.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_35C_SEQUENTIAL_GRAPH_Trial2.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_45C_GRAPH_Trial2.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_45C_SEQUENTIAL_GRAPH_Trial2.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_55C_GRAPH_Trial2.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_55C_SEQUENTIAL_GRAPH_Trial2.png|750px]] | |||
<b>Trial 3 Data:</b> | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_5C_GRAPH_Trial3.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_5C_SEQUENTIAL_GRAPH_Trial3.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_15C_GRAPH_Trial3.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_15C_SEQUENTIAL_GRAPH_Trial3.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_25C_GRAPH_Trial3.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_25C_SEQUENTIAL_GRAPH_Trial3.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_35C_GRAPH_Trial3.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_35C_SEQUENTIAL_GRAPH_Trial3.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_45C_GRAPH_Trial3.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_45C_SEQUENTIAL_GRAPH_Trial3.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_55C_GRAPH_Trial3.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_55C_SEQUENTIAL_GRAPH_Trial3.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_65C_GRAPH_Trial3.png|750px]] | |||
[[Image:Mb_Acetate_OPD_H2O2_H2O_65C_SEQUENTIAL_GRAPH_Trial3.png|750px]] | |||
== Synthesis of Buffered Mb == | |||
This synthesis was taken from [http://openwetware.org/wiki/User:Paul_Rothenberg/Notebook/Pauls_Notebook/2013/05/22 May 22]. | |||
5 mg of Mb and approximately 490-500 milligrams of KCl were added to four tubes, and 20 mL of pH 3 Citrate, pH 5 Acetate, pH 7 Phosphate, and pH 9 Tris buffers were added. | |||
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Latest revision as of 22:53, 26 September 2017
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Reaction of OPD+H2O2 with Mb/Acetate Catalyst in H2OGoing to continue the trials in H2O with Mb/Acetate. We are also going to make up more buffered protein solutions. Trial 2 Data:
Trial 3 Data:
Synthesis of Buffered MbThis synthesis was taken from May 22. 5 mg of Mb and approximately 490-500 milligrams of KCl were added to four tubes, and 20 mL of pH 3 Citrate, pH 5 Acetate, pH 7 Phosphate, and pH 9 Tris buffers were added. | |
