User:Paul Rothenberg/Notebook/Pauls Notebook/2014/03/05: Difference between revisions
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==Heme Extraction from Myoglobin== | ==Heme Extraction from Myoglobin== | ||
* Preparation of Apoprotein: | * Preparation of Apoprotein: | ||
#24. | #24.3 mg of Tris was added to 4 mL of water to make a 0.05M solution to dissolve 200 mg of myoglobin | ||
# 3.36 g of Sodium Bicarbonate was dissolved in 4L of H<sub>2</sub>O to make a 10 mM dialysis buffer | |||
#The pH of the Tris solution was brought down to 2.3-2.5 at 0°C before 2-butanone extraction was performed | |||
#2-butanone was placed on ice for the extraction | |||
#Acid/2-butanone extraction was performed on the protein solution with equal parts 2-butanone and protein | |||
#The aqueous extract was then added to a dialysis cassette and placed in the dialysis buffer in the 0°C cold room and will sit overnight | |||
##Approximately 3 mL of aqueous extract was extracted overall | |||
Revision as of 11:32, 5 March 2014
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Heme Extraction from Myoglobin
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