User:Paul Rothenberg/Notebook/Pauls Notebook/2014/03/05: Difference between revisions
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#24.3 mg of Tris was added to 4 mL of water to make a 0.05M solution to dissolve 200 mg of myoglobin | #24.3 mg of Tris was added to 4 mL of water to make a 0.05M solution to dissolve 200 mg of myoglobin | ||
# 3.36 g of Sodium Bicarbonate was dissolved in 4L of H<sub>2</sub>O to make a 10 mM dialysis buffer | # 3.36 g of Sodium Bicarbonate was dissolved in 4L of H<sub>2</sub>O to make a 10 mM dialysis buffer | ||
# | #The pH of the Tris solution was brought down to 2.3-2.5 at 0°C before 2-butanone extraction was performed. | ||
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Heme Extraction from Myoglobin
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