User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/02

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(Preparation of Apo-Mb)
(Synthesis of Cobalt PPIX)
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==Synthesis of Cobalt PPIX==
==Synthesis of Cobalt PPIX==
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Stoichiometric amounts of Cobalt Acetate (524 mg) were measured out, along with 500 mg of PPIX. 50 mL of DMF was set to reflux. Once refluxing, the cobalt acetate and PPIX were dissolved in minimum DMF and added via syringe to the refluxing DMF.
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Revision as of 13:11, 2 June 2014

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Preparation of Apo-Mb

I am following the same procedure as here to prepare Apomyoglobin.

The heme was extracted using the acid and 2-butanone method. Approximately 10 mL of the aqueous layer was produced. It was left to run dialysis overnight at 4°C in the 10 mM Na2CO3 buffer.

Synthesis of Cobalt PPIX

Stoichiometric amounts of Cobalt Acetate (524 mg) were measured out, along with 500 mg of PPIX. 50 mL of DMF was set to reflux. Once refluxing, the cobalt acetate and PPIX were dissolved in minimum DMF and added via syringe to the refluxing DMF.


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