User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/03: Difference between revisions
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==Addition of Mn PPIX to Apo-Mb synthesized yesterday== | ==Addition of Mn PPIX to Apo-Mb synthesized yesterday== | ||
Andrew and I are going to reconstitute the Mn-PPIX into the apo-Mb we prepared and left to run dialysis yesterday. For this, we are using the same protocol I ran a couple of months ago (link needs to be inserted). | Andrew and I are going to reconstitute the Mn-PPIX into the apo-Mb we prepared and left to run dialysis yesterday. For this, we are using the same protocol I ran a couple of months ago (link needs to be inserted). Assuming a complete conversion of the protein, we have 500 mg of apoprotein in solution. Since it's 2 mg PPIX per 10 mg of apoprotein, we will use 100 mg of PPIX. | ||
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Addition of Mn PPIX to Apo-Mb synthesized yesterdayAndrew and I are going to reconstitute the Mn-PPIX into the apo-Mb we prepared and left to run dialysis yesterday. For this, we are using the same protocol I ran a couple of months ago (link needs to be inserted). Assuming a complete conversion of the protein, we have 500 mg of apoprotein in solution. Since it's 2 mg PPIX per 10 mg of apoprotein, we will use 100 mg of PPIX.
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