User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/03

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Entry title)
(Addition of Mn PPIX to Apo-Mb synthesized yesterday)
Line 8: Line 8:
==Addition of Mn PPIX to Apo-Mb synthesized yesterday==
==Addition of Mn PPIX to Apo-Mb synthesized yesterday==
-
Andrew and I are going to reconstitute the Mn-PPIX into the apo-Mb we prepared and left to run dialysis yesterday. For this, we are using the same protocol I ran a couple of months ago (link needs to be inserted).  
+
Andrew and I are going to reconstitute the Mn-PPIX into the apo-Mb we prepared and left to run dialysis yesterday. For this, we are using the same protocol I ran a couple of months ago (link needs to be inserted). Assuming a complete conversion of the protein, we have 500 mg of apoprotein in solution. Since it's 2 mg PPIX per 10 mg of apoprotein, we will use 100 mg of PPIX.  

Revision as of 08:42, 3 June 2014

Project name Main project page
Previous entry      Next entry

Addition of Mn PPIX to Apo-Mb synthesized yesterday

Andrew and I are going to reconstitute the Mn-PPIX into the apo-Mb we prepared and left to run dialysis yesterday. For this, we are using the same protocol I ran a couple of months ago (link needs to be inserted). Assuming a complete conversion of the protein, we have 500 mg of apoprotein in solution. Since it's 2 mg PPIX per 10 mg of apoprotein, we will use 100 mg of PPIX.



Personal tools