User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/03

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(Addition of Mn PPIX to Apo-Mb synthesized yesterday)
(Addition of Mn PPIX to Apo-Mb synthesized yesterday)
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==Addition of Mn PPIX to Apo-Mb synthesized yesterday==
==Addition of Mn PPIX to Apo-Mb synthesized yesterday==
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Andrew and I are going to reconstitute the Mn-PPIX into the apo-Mb we prepared and left to run dialysis yesterday. For this, we are using the same protocol I ran a couple of months ago (link needs to be inserted). Assuming a complete conversion of the protein, we have 500 mg of apoprotein in solution. Since it's 2 mg PPIX per 10 mg of apoprotein, we will use 100 mg of PPIX.  
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Andrew and I are going to reconstitute the Mn-PPIX into the apo-Mb we prepared and left to run dialysis yesterday. For this, we are using the same protocol I ran a couple of months ago (link needs to be inserted). Assuming a complete conversion of the protein, we have 500 mg of apoprotein in solution. Since it's 2 mg PPIX per 10 mg of apoprotein, we will use 50mg of PPIX because we did not run all of the apoprotein through dialysis. We have the rest in the fridge that we can use later.

Revision as of 07:49, 3 June 2014

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Addition of Mn PPIX to Apo-Mb synthesized yesterday

Andrew and I are going to reconstitute the Mn-PPIX into the apo-Mb we prepared and left to run dialysis yesterday. For this, we are using the same protocol I ran a couple of months ago (link needs to be inserted). Assuming a complete conversion of the protein, we have 500 mg of apoprotein in solution. Since it's 2 mg PPIX per 10 mg of apoprotein, we will use 50mg of PPIX because we did not run all of the apoprotein through dialysis. We have the rest in the fridge that we can use later.



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