User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/04: Difference between revisions
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== | ==Mb Mn-PPIX Purification== | ||
Dr. Hartings, Andrew, and I attempted to purify the Mn-Mb I made during the semester. We used the 100 mM Phosphate buffers, and everything eluted through the column. So, Andrew and I made 50 mM Tris buffer and 50 mM Tris/1M NaCl buffer. 6.057g of Tris was added to each, and 1 mol of NaCl (58.44g) were added, 900 mL of H<sub>2</sub>O were added, and titrated to pH 9 with 3M HCl. The remaining volume was added, each solution was filtered with a fritted funnel, and autoclaved. The purification will be run tomorrow. | |||
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Mb Mn-PPIX PurificationDr. Hartings, Andrew, and I attempted to purify the Mn-Mb I made during the semester. We used the 100 mM Phosphate buffers, and everything eluted through the column. So, Andrew and I made 50 mM Tris buffer and 50 mM Tris/1M NaCl buffer. 6.057g of Tris was added to each, and 1 mol of NaCl (58.44g) were added, 900 mL of H2O were added, and titrated to pH 9 with 3M HCl. The remaining volume was added, each solution was filtered with a fritted funnel, and autoclaved. The purification will be run tomorrow.
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