User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/10: Difference between revisions
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== | ==Purification of Mn-Mb== | ||
Going to run the Mn-Mb we made through a PD-10 column with H<sub>2</sub>O. We will run 25 mL of H<sub>2</sub>O through to equilibrate the column, and let that run through. Following that, we will add 2.5 mL of protein, and let the band collect at the top. Then, 3.5 mL of H<sub>2</sub>O will be added, and the band collected. Following collection, 25 mL of H<sub>2</sub>O will be run through, and that procedure will be repeated until we have collected all the protein. | |||
Revision as of 05:43, 10 June 2014
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Purification of Mn-MbGoing to run the Mn-Mb we made through a PD-10 column with H2O. We will run 25 mL of H2O through to equilibrate the column, and let that run through. Following that, we will add 2.5 mL of protein, and let the band collect at the top. Then, 3.5 mL of H2O will be added, and the band collected. Following collection, 25 mL of H2O will be run through, and that procedure will be repeated until we have collected all the protein.
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