User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/11

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==Entry title==
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==Synthesis of Cobalt Mb==
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Continuing the synthesis of Cobalt Mb. Added 100 mg of Co-PPIX to 2 mL of autoclaved 100 mM Phosphate buffer, along with 10 drops of 0.1 N NaOH. This was left to cool to 4°C. Following, it was added dropwise to the apoprotein and let react overnight.  

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Synthesis of Cobalt Mb

Continuing the synthesis of Cobalt Mb. Added 100 mg of Co-PPIX to 2 mL of autoclaved 100 mM Phosphate buffer, along with 10 drops of 0.1 N NaOH. This was left to cool to 4°C. Following, it was added dropwise to the apoprotein and let react overnight.



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