User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/27: Difference between revisions
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'''Results''' | '''Results''' | ||
[[Image:Mb_Phosphate_OPD_H2O2_MeCN_25C_Graph.png]] | [[Image:Mb_Phosphate_OPD_H2O2_MeCN_25C_Graph.png|500px]] | ||
Revision as of 17:29, 28 June 2014
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PurificationAndrew and I finished purifying both the Apo Mb and the Mn Mb in the PD10 columns. The Mn Mb resulted in roughly 30 mL of liquid. 15 mL was removed, frozen, and lyophilized. The other 15 mL had 1.5 g of KCl dissolved in it, frozen, diluted with 5 mL of Phosphate buffer to 20 mL, and lyophilized. The Apo Mb was held because the Co PPIX did not go into solution fully. Mb Phosphate in MeCNThis reaction was run at 25°C. The results are below: Results
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