User:Paul Rothenberg/Notebook/Pauls Notebook/2014/06/27: Difference between revisions
From OpenWetWare
| Line 9: | Line 9: | ||
Andrew and I finished purifying both the Apo Mb and the Mn Mb in the PD10 columns. The Mn Mb resulted in roughly 30 mL of liquid. 15 mL was removed, frozen, and lyophilized. The other 15 mL had 1.5 g of KCl dissolved in it, frozen, diluted with 5 mL of Phosphate buffer to 20 mL, and lyophilized. | Andrew and I finished purifying both the Apo Mb and the Mn Mb in the PD10 columns. The Mn Mb resulted in roughly 30 mL of liquid. 15 mL was removed, frozen, and lyophilized. The other 15 mL had 1.5 g of KCl dissolved in it, frozen, diluted with 5 mL of Phosphate buffer to 20 mL, and lyophilized. | ||
==Preparation of Co Mb== | |||
10 mg of Co PPIX was dissolved in 2 mL of 100 mM Phosphate buffer with 15 drops of 0.1 N NaOH. This was left to stir over the weekend to go into solution. The apoprotein will be reconstituted on Monday. | |||
==Mb Phosphate in MeCN== | ==Mb Phosphate in MeCN== | ||
Revision as of 17:31, 28 June 2014
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
PurificationAndrew and I finished purifying both the Apo Mb and the Mn Mb in the PD10 columns. The Mn Mb resulted in roughly 30 mL of liquid. 15 mL was removed, frozen, and lyophilized. The other 15 mL had 1.5 g of KCl dissolved in it, frozen, diluted with 5 mL of Phosphate buffer to 20 mL, and lyophilized. Preparation of Co Mb10 mg of Co PPIX was dissolved in 2 mL of 100 mM Phosphate buffer with 15 drops of 0.1 N NaOH. This was left to stir over the weekend to go into solution. The apoprotein will be reconstituted on Monday. Mb Phosphate in MeCNThis reaction was run at 25°C. The results are below: Results
| |
