User:Paul Rothenberg/Notebook/Pauls Notebook/2014/07/01: Difference between revisions
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== | ==Testing Control Reactions== | ||
Before moving on any further, we ran control reactions in different solvents to see if heme or the protein has catalytic properties. We made a stock of hemin in 5 mL of solvent, along with 10 mg of Mb Citrate and Phosphate in 1 mL of solvent. We took spectra of these and determined how much each needed to be diluted to have an A(400) of 0.1. Following this, the diluted solutions were prepared. OPD and Peroxide solutions were made, and added to 4 tubes each. The four reactions were as follows: | |||
#Control - no protein or hemin | |||
#Hemin only | |||
#Mb Citrate | |||
#Mb Phosphate | |||
These were prepared and let react for 2 hours on the shake plate. | |||
Revision as of 09:04, 1 July 2014
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Testing Control ReactionsBefore moving on any further, we ran control reactions in different solvents to see if heme or the protein has catalytic properties. We made a stock of hemin in 5 mL of solvent, along with 10 mg of Mb Citrate and Phosphate in 1 mL of solvent. We took spectra of these and determined how much each needed to be diluted to have an A(400) of 0.1. Following this, the diluted solutions were prepared. OPD and Peroxide solutions were made, and added to 4 tubes each. The four reactions were as follows:
These were prepared and let react for 2 hours on the shake plate.
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