User:Paul Rothenberg/Notebook/Pauls Notebook/2014/07/01: Difference between revisions
From OpenWetWare
| Line 20: | Line 20: | ||
[[Image:Control_Reaction_IsOH_120Min_Chart.png|500px]] | [[Image:Control_Reaction_IsOH_120Min_Chart.png|500px]] | ||
[[Image:EtOH_Control_120Min_Chart.png|500px]] | |||
Revision as of 12:37, 1 July 2014
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Testing Control ReactionsBefore moving on any further, we ran control reactions in different solvents to see if heme or the protein has catalytic properties. We made a stock of hemin in 5 mL of solvent, along with 10 mg of Mb Citrate and Phosphate in 1 mL of solvent. We took spectra of these and determined how much each needed to be diluted to have an A(400) of 0.1. Following this, the diluted solutions were prepared. OPD and Peroxide solutions were made, and added to 4 tubes each. The four reactions were as follows:
The solvents used were Isopropanol and Ethanol. These were prepared and let react for 2 hours on the shake plate. Results
| |
