User:Paula Satisteban Vela: Difference between revisions
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[[Image:Paula.jpg|thumb|right|Paula | [[Image:Paula.jpg|thumb|right|Paula Santisteban Vela (an artistic interpretation)]] | ||
*Paula Santisteban Vela | *Nury Paula Santisteban Vela | ||
* | *Memorial University of Newfoundland | ||
* | *St. John's, NL, Canada | ||
* | *nps071@mun.ca | ||
==Education== | ==Education== | ||
<!--Include info about your educational background--> | <!--Include info about your educational background--> | ||
* | * 2019 Ph.D., Memorial University of Newfoundland, NL, Canada | ||
* 2010, | '''Thesis title:''' Interaction of Antimicrobial Peptides with the Gram Positive and Gram Negative Cell Envelope <br> | ||
'''Supervisor:''' Valerie Booth. Co-Supervisor: Michael Morrow <br> | |||
**During my time as a Ph.D. student, I developed and tested protocols for growing deuterium-rich bacteria to be able to perform deuterium NMR measurements in whole cells. I also tested the changes in the <sup>2</sup>H NMR spectra of ''E. coli'' and ''B. subtilis '' in the presence of Antimicrobial peptides. Finally, I compared the <sup>2</sup>H NMR spectra of ''B. subtilis'' with intact and compromised peptidoglycan layers. | |||
* 2013 MSc, Universidad De Los Andes, Bogotá | |||
'''Thesis title:''' Mechanical properties of membranes as a factor of resistance to Antimicrobial Peptides <br> | |||
'''Supervisor:''' Chad Leidy <br> | |||
* 2010, BSc, Universidad Nacional De Colombia | |||
==Research interests== | ==Research interests== | ||
| Line 19: | Line 23: | ||
# Biophysics | # Biophysics | ||
# Mechanics of Membrane | # Mechanics of Membrane | ||
# NMR | |||
#Antimicrobial Peptides | |||
#NMR in whole cell | |||
==Publications== | ==Publications== | ||
<!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications. You can add or remove lines as needed --> | <!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications. You can add or remove lines as needed --> | ||
<biblio> | <biblio> | ||
#Paper1 pmid= | #Paper1 pmid=31987866 | ||
#Paper2 pmid= | #Paper2 pmid=28844739 | ||
#Paper3 pmid=28013507 | |||
// leave a comment about a paper here | // leave a comment about a paper here | ||
</biblio> | </biblio> | ||
==Events Participation== | |||
# Unraveling the Role of Peptidoglycan in the Interaction of Antimicrobial Peptides BP100 and MSI-78 with Bacterial Cell Envelopes through <sup>2</sup>H NMR Experiments,''Biophysical Journal'', VOLUME 114, ISSUE 3, SUPPLEMENT 1, 454A, FEBRUARY 02, 2018. [https://doi.org/10.1016/j.bpj.2017.11.2508] | |||
# <sup>2</sup>H NMR Studies of Bacteria: How Does the Peptidoglycan Layer Modify the Interaction between Antimicrobial Peptides and the Lipid Membrane,''Biophysical Journal'', VOLUME 112, ISSUE 3, SUPPLEMENT 1, 380A-381A, FEBRUARY 03, 2017. [https://doi.org/10.1016/j.bpj.2016.11.2068] | |||
# <sup>2</sup>H NMR Studies of Living Bacteria Interacting with Antimicrobial Peptides,''Biophysical Journal'', VOLUME 110, ISSUE 3, SUPPLEMENT 1, 416A-417A, FEBRUARY 16, 2016. [https://doi.org/10.1016/j.bpj.2015.11.2252] | |||
# The Antimicrobial Peptide Analog Magainin-H2 Induces Changes in the Phase Behavior and Lateral Organization of Supported Bilayers During Liquid-Crystalline/Gel Phase Coexistence,''Biophysical Journal'', VOLUME 102, ISSUE 3, SUPPLEMENT 1, 93A, JANUARY 31, 2012. [https://doi.org/10.1016/j.bpj.2011.11.526] | |||
==Useful links== | ==Useful links== | ||
*[[OpenWetWare:Welcome|Introductory tutorial]] | *[[OpenWetWare:Welcome|Introductory tutorial]] | ||
*[[Help|OpenWetWare help pages]] | *[[Help|OpenWetWare help pages]] | ||
Latest revision as of 13:10, 5 May 2020
- Nury Paula Santisteban Vela
- Memorial University of Newfoundland
- St. John's, NL, Canada
- nps071@mun.ca
Education
- 2019 Ph.D., Memorial University of Newfoundland, NL, Canada
Thesis title: Interaction of Antimicrobial Peptides with the Gram Positive and Gram Negative Cell Envelope
Supervisor: Valerie Booth. Co-Supervisor: Michael Morrow
- During my time as a Ph.D. student, I developed and tested protocols for growing deuterium-rich bacteria to be able to perform deuterium NMR measurements in whole cells. I also tested the changes in the 2H NMR spectra of E. coli and B. subtilis in the presence of Antimicrobial peptides. Finally, I compared the 2H NMR spectra of B. subtilis with intact and compromised peptidoglycan layers.
- 2013 MSc, Universidad De Los Andes, Bogotá
Thesis title: Mechanical properties of membranes as a factor of resistance to Antimicrobial Peptides
Supervisor: Chad Leidy
- 2010, BSc, Universidad Nacional De Colombia
Research interests
- Biophysics
- Mechanics of Membrane
- NMR
- Antimicrobial Peptides
- NMR in whole cell
Publications
- Santisteban NP, Morrow MR, and Booth V. Effect of AMPs MSI-78 and BP100 on the lipid acyl chains of (2)H-labeled intact Gram positive bacteria. Biochim Biophys Acta Biomembr. 2020 May 1;1862(5):183199. DOI:10.1016/j.bbamem.2020.183199 |
- Booth V, Warschawski DE, Santisteban NP, Laadhari M, and Marcotte I. Recent progress on the application of (2)H solid-state NMR to probe the interaction of antimicrobial peptides with intact bacteria. Biochim Biophys Acta Proteins Proteom. 2017 Nov;1865(11 Pt B):1500-1511. DOI:10.1016/j.bbapap.2017.07.018 |
- Santisteban NP, Morrow MR, and Booth V. Protocols for Studying the Interaction of MSI-78 with the Membranes of Whole Gram-Positive and Gram-Negative Bacteria by NMR. Methods Mol Biol. 2017;1548:217-230. DOI:10.1007/978-1-4939-6737-7_15 |
leave a comment about a paper here
Events Participation
- Unraveling the Role of Peptidoglycan in the Interaction of Antimicrobial Peptides BP100 and MSI-78 with Bacterial Cell Envelopes through 2H NMR Experiments,Biophysical Journal, VOLUME 114, ISSUE 3, SUPPLEMENT 1, 454A, FEBRUARY 02, 2018. [1]
- 2H NMR Studies of Bacteria: How Does the Peptidoglycan Layer Modify the Interaction between Antimicrobial Peptides and the Lipid Membrane,Biophysical Journal, VOLUME 112, ISSUE 3, SUPPLEMENT 1, 380A-381A, FEBRUARY 03, 2017. [2]
- 2H NMR Studies of Living Bacteria Interacting with Antimicrobial Peptides,Biophysical Journal, VOLUME 110, ISSUE 3, SUPPLEMENT 1, 416A-417A, FEBRUARY 16, 2016. [3]
- The Antimicrobial Peptide Analog Magainin-H2 Induces Changes in the Phase Behavior and Lateral Organization of Supported Bilayers During Liquid-Crystalline/Gel Phase Coexistence,Biophysical Journal, VOLUME 102, ISSUE 3, SUPPLEMENT 1, 93A, JANUARY 31, 2012. [4]
