User:Paulina Alatriste/Notebook/UNAM Genomics Mexico 2011/2011/08/17
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LB media preparation/ Calculation of oligo dilutionsABSTRACT
-When the medium is solid I added 15 g/L of bacteriologic agar.
I used a formula that says C0V0=CFVF where C0 is initial concentration, V0 is initial volume, CF is final concentration and VF is final volume. We want a final concentration of 5pM/μL and a final volume of 500μL. So by knowing the initial concentration we can use this formula to find the volume of oligo and water that has to be mixed to obtain the desired concentration.
-C0= 341.02 pM/mL -Clearing for V0 we obtain that he have to mix 7 μL of oligo and 493 of water.
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