User:Pauline Wonnenberg/Notebook/CHEM 471 Fall 2017/2017/08/30: Difference between revisions

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<br>Following the above table, here is the UV-Vis spectrometer collected analyzing the samples of Phenylalanine.<br>[[Image:08-30_Phenylalanine.png]]<br>
<br>Following the above table, here is the UV-Vis spectrometer collected analyzing the samples of Phenylalanine.<br>[[Image:08-30_Phenylalanine.png|400px]]<br>
<table class="demo">
<table class="demo">
<caption>Amine - Arg</caption>
<caption>Amine - Arg</caption>
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<br>Following the above table, here is the UV-Vis spectrometer collected analyzing the samples of Arginine.<br>[[Image:08-30_Amine_Graph.png]]<br>
<br>Following the above table, here is the UV-Vis spectrometer collected analyzing the samples of Arginine.<br>[[Image:08-30_Amine_Graph.png|400px]]<br>
<table class="demo">
<table class="demo">
<caption>Aliphatic - Iso</caption>
<caption>Aliphatic - Iso</caption>
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<br>Following the above table, here is the UV-Vis spectrometer collected analyzing the samples of Isoleucine.<br>[[Image:08-30_Isoleucine_Graph.png]]<br>
<br>Following the above table, here is the UV-Vis spectrometer collected analyzing the samples of Isoleucine.<br>[[Image:08-30_Isoleucine_Graph.png|400px]]<br>


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Revision as of 12:02, 19 September 2017

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Laboratory 2

  • AuNP not formed during previous laboratory; trial 2 for solutions below:


Power of ten error was found, see updated calculations below:
Goal: 2.5mM HAuCl4 into 50mL flask
Calculations: 0.05L x 2.5x10-3M = 1.25x10-4moles
HAuCl4 = 339.785g/mol
339.785g/mol : 1.25x10-4moles = 0.04247g = 42.47mg

  • Preparation of 3 different amino acid solutions with the same concentration as BSA (250 μM):


Choice of amino acids: Allopathic - Isoleucine; Aromatic - Phenylalanine; Amine - Arginine
Isoleucine Calculations: 0.05L x 250x10-6M = 1.25x10-5moles
Molar mass of Isoleucine: 131.18g/mol
131.18g/mol x 1.25x10-5M = 0.001639grams
Phenylalanine Calculations: 0.05L x 250x10-6M = 1.25x10-5moles
Molar mass of Phenylalanine: 165.19g/mol
165.19g/mol x 1.25x10-5M = 0.002065grams
Arginine Calculations: 0.05L x 250x10-6M = 1.25x10-5moles
Molar mass of Arginine: 174.20g/mol
174.20g/mol x 1.25x10-5M = 0.0021775grams

  • pH Solutions Measurements:


Aromatic - Phe
Ratio pH Values
 20 3.327
 80 3.273 
 160 3.244 


Following the above table, here is the UV-Vis spectrometer collected analyzing the samples of Phenylalanine.

Amine - Arg
Ratio pH Values
 20 3.372
 80 3.260 
 160 3.272 


Following the above table, here is the UV-Vis spectrometer collected analyzing the samples of Arginine.

Aliphatic - Iso
Ratio pH Values
 20 3.235
 80 3.292
 160 3.338 


Following the above table, here is the UV-Vis spectrometer collected analyzing the samples of Isoleucine.
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