User:Pedrobeltrao/Notebook/Structural analysis of phosphorylation sites/domain based analysis
Pedrobeltrao (Talk | contribs)
(New page: ==Domain based analysis== Phosphorylation sites from S. cerevisiae were collected from different sources . In addition phosphorylation sites from H. sapiens, D. melanogaster, C. albicans ...)
Next diff →
Revision as of 17:32, 23 November 2009
Domain based analysis
Phosphorylation sites from S. cerevisiae were collected from different sources . In addition phosphorylation sites from H. sapiens, D. melanogaster, C. albicans and S. pombe were transfered by homology to S. cerevisiae proteins by aligning S. cerevisiae proteins with orthologous proteins in these species. Up to date values for phospho-sites can be seen [here]).
One possible type of analysis that can be done with this information is to predict modes of regulation for protein domains that are commonly phosphorylated. For each PFAM domain we can we have picked an example structure to use as a structural model. We then mapped all phosphorylation sites that occur in instances of these domain in S. cerevisiae or orthologous proteins to this model structure and calculated phosphorylation propensity . As an example we show below the results obtained for the protein kinase domain.