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| == Protocols == | | == Protocols == |
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| ===Edited Protocols== | | ===Edited Protocols=== |
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| Fluorometer- Optimum Exposure Time
| | [[GFP vs Fluorescence Calibration Curve]]<br> |
| Volume dependance of Fluorescence
| | [[Purification of LuxR]]<br> |
| GFP vs Fluorescence Calibration Curve | | ====Cell By Date==== |
| Degradation Time of GFP
| | [[Initial Testing for DNA constructs In Vivo]]<br> |
| Purification of LuxR | | [[Initial Testing for DNA constructs In Vitro]]<br> |
| | [[Operating Temperature Range (Initial Testing)]] <br> |
| | [[Operating Temperature Range (Refined Testing)]] |
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| | | ====Infector Detector==== |
| | | [[Initial Testing of DNA Construct In Vivo]]<br> |
| Our cool new layout!
| | [[Initial Testing of DNA Construct In Vitro]]<br> |
| | | [[Preliminary AHL Testing]]<br> |
| {| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left"
| | [[AHL Sensitivity: Real Life Applications]]<br> |
| |- style="background:#aabadd; color:Black" align="center"
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| || <font size=3>'''Fabrication Protocols'''</font>|| <font size=3>'''Testing Protocols'''</font>
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| |- style="background:#d5dcef; color:Black" valign="top"
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| <font size=3>'''General Protocols'''</font> <br>
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/DNA1.1|Cloning of DNA- MiniPrep]]
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/DNA1.2|Cloning of DNA- MidiPrep]]
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/DNA1.3|Cloning of DNA- MaxiPrep]]
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.6|Purification of GFP]]
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.1|Fluorometer- Optimum Exposure Time]]
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.2|Volume dependance of Fluorescence]]
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.3|GFP vs Fluorescence Calibration Curve]]
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.4|Degradation Time of GFP]]
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.5|Purification of LuxR]]
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| <br>
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| <font size=3>'''Chassis Protocols'''</font> <br>
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| '''''E. coli'' Extracts'''<br>
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.1|Preparation of S30 ''E. coli'' extract]]
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.2|Preparation of S12 ''E. coli'' extract]]
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CE1.3|Preparation of reaction mixture]]
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| '''Vesicles Formation'''<br>
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| * 2
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| <font size=3>'''Cell by Date'''</font> <br>
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| '''Phase 1'''<br>
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD1.1|Initial Testing for DNA constructs of CBD In Vivo]]
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD1.2|Initial Testing for DNA constructs of CBD In Vitro]]
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD1.3|Problems with Evaporation over a 24 hour period]]
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| '''Phase 2'''<br>
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD2.1|Operating Temperature Range (Initial Tests)]]
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/CBD2.2|Operating Temperature Range (Refined Tests)]]
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| '''Phase 3'''<br>
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| <br>
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| <font size=3>'''Infector Detector'''</font> <br>
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| '''Phase 1'''<br>
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID1.1|Initial Testing for DNA constructs of ID In Vivo]]
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID1.2|Initial Testing for DNA constructs of ID In Vitro]]
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| '''Phase 2'''<br>
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID2.1|Preliminary AHL Testing]]
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| * [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/ID2.2|AHL Sensitivity: Real Life Applications]]
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| '''Phase 3'''<br>
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| * 2
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| |}
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