User:Perry/Spring 2007

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Revision as of 08:33, 13 February 2007 by Perry (talk | contribs) (New page: ==2/12/07== dissolved 500mg ampicillin salt into 10ml water to prepare 50mg/ml ampicillin stock; aliquotted 1ml into tubes, left in -20dC. overnight 1ml LB-amp cultures of JLO159, W, H, S...)
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2/12/07

dissolved 500mg ampicillin salt into 10ml water to prepare 50mg/ml ampicillin stock; aliquotted 1ml into tubes, left in -20dC.

overnight 1ml LB-amp cultures of JLO159, W, H, S, ~4:30pm.

2/13/07

took overnight cultures out ~7:30pm. diluted 1:10, measured OD600.

159- .335, W- .245, H- .231, S- .279

made liquid cultures of 5ml LB-amp, 250ul overnight cultures. incubated 3h, added 5mM IPTG (53ul 0.5M).

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