User:Philip Romero/Notebook/P450 chimera construction/2008/04/13

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(Autocreate 2008/04/13 Entry for User:Philip_Romero/Notebook/P450_chimera_construction)
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=Chimera cloning=
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*cloning the Otey 3-block chimeras into the pET-22b plasmid
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*currently have all 17 chimeras in pCWori miniprepped
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*need to PCR, digest, extract, ligate, transform, and plate
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Revision as of 19:18, 14 April 2008

Project name Main project page
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Chimera cloning

  • cloning the Otey 3-block chimeras into the pET-22b plasmid
  • currently have all 17 chimeras in pCWori miniprepped
  • need to PCR, digest, extract, ligate, transform, and plate


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