User:Philip Romero/Notebook/P450 chimera construction/2008/04/13: Difference between revisions

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(Autocreate 2008/04/13 Entry for User:Philip_Romero/Notebook/P450_chimera_construction)
 
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==Entry title==
==Entry title==
* Insert content here...
=Chimera cloning=
*cloning the Otey 3-block chimeras into the pET-22b plasmid
*currently have all 17 chimeras in pCWori miniprepped
*need to PCR, digest, extract, ligate, transform, and plate
 
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Revision as of 17:18, 14 April 2008

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Entry title

Chimera cloning

  • cloning the Otey 3-block chimeras into the pET-22b plasmid
  • currently have all 17 chimeras in pCWori miniprepped
  • need to PCR, digest, extract, ligate, transform, and plate