User:Philip Romero/Notebook/P450 chimera construction/2008/04/13

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Chimera cloning

  • cloning the Otey 3-block chimeras into the pET-22b plasmid
  • currently have all 17 chimeras in pCWori miniprepped
  • need to PCR, digest, extract, ligate, transform, and plate


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Philip_Romero/Notebook/P450_chimera_construction/2008/04/13&oldid=196638"