User:Phillip R. Smith: Difference between revisions

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*[http://www.attotron.com/cybertory/analysis/trans.htm DNA<->RNA->Protein]
*[http://www.attotron.com/cybertory/analysis/trans.htm DNA<->RNA->Protein]
*[http://www.encorbio.com/protocols/Nuc-MW.htm DNA MW Calculator]
*[http://www.encorbio.com/protocols/Nuc-MW.htm DNA MW Calculator]
*[http://www.bioinformatics.org/sms/prot_mw.html Protein MW Calculator]
*[http://www.liv.ac.uk/buffers/buffercalc.html Buffer Calculator]
*[http://www.liv.ac.uk/buffers/buffercalc.html Buffer Calculator]
*[http://robetta.bakerlab.org/ Robetta]
*[http://robetta.bakerlab.org/ Robetta]

Revision as of 12:32, 28 November 2011

Contact Info

Phillip R. Smith

<html><a href="http://www.mendeley.com/profiles/phillip-smith2/"><img border="0" src="http://www.mendeley.com/embed/icon/1/red/small" alt="Research papers by Phillip Smith"/></a></html> <html><a href="http://www.linkedin.com/pub/phil-smith/7/626/674" >

         <img src="http://www.linkedin.com/img/webpromo/btn_viewmy_120x33.png" width="120" height="33" border="0" alt="View Phil Smith's profile on LinkedIn">
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Education

  • 2005-Present, PhD Candidate, Chemical Engineering, Stanford University
  • 2005, MS, Chemical Engineering, Brigham Young University
  • 2003, BS, Chemical Engineering, Brigham Young University

Papers

Bingham AS, Smith PR, Swartz JR. Evolution of an [FeFe] hydrogenase with decreased oxygen sensitivity. International Journal of Hydrogen Energy (2011) doi:10.1016/j.ijhydene.2011.02.048

Smith PR, Bingham AS, Swartz JR. Generation of hydrogen from NADPH using an [FeFe] hydrogenase. International Journal of Hydrogen Energy (2011) doi:10.1016/j.ijhydene.2011.03.172

Technical Presentations

Research interests

  1. Protein Biotechnology (molecular engineering and directed protein evolution)
  2. Biochemical Reaction Engineering (bioprocess development)
  3. Synthetic Enzyme Pathways

Biotechnology skillset

  • Protein overexpression (aerobic and anaerobic fermentation, shake-flask to 10L scale)
  • Protein purification (ion-exchange, affinity, and other chromatographic methods using an AKTA purifier and home-made systems)
  • Directed enzyme evolution
  • Protein engineering
  • PCR, gene synthesis, mutagenesis
  • Spectrophotometric assay development

Useful links

Worth checking out