User:Pranav Rathi/Notebook/OT/2010/11/11/Small Bead Power Spectrum & DOG - Revision history

Pranav Rathi at 20:51, 15 November 2012

Pranav Rathi — Thu, 15 Nov 2012 20:51:00 GMT

←Older revision		Revision as of 20:51, 15 November 2012
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	== Power Spectrum ==		== Power Spectrum ==
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-	[[Category:Optical ~~Tweezer~~]]	+	[[Category:Optical tweezers Notes Archive]]

Pranav Rathi at 23:42, 11 February 2011

Pranav Rathi — Fri, 11 Feb 2011 23:42:51 GMT

←Older revision		Revision as of 23:42, 11 February 2011
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-	~~{{ShowGooglePowerpoint\|v&pid=explore=0B5jWjFYiQdkfODYzNzUxN2EtNzlhZi00NjA3LWI1ZTQtN2Q4M2ViOTFjOTIy~~	+
-	~~\|size=medium}}~~	+

	== Power Spectrum ==		== Power Spectrum ==

Pranav Rathi: /* Power Spectrum */

Pranav Rathi — Fri, 11 Feb 2011 23:38:48 GMT

Power Spectrum

←Older revision		Revision as of 23:38, 11 February 2011
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		+	{{ShowGooglePowerpoint\|v&pid=explore=0B5jWjFYiQdkfODYzNzUxN2EtNzlhZi00NjA3LWI1ZTQtN2Q4M2ViOTFjOTIy
		+	\|size=medium}}
		+
	== Power Spectrum ==		== Power Spectrum ==
	I have done power spectrum analysis for small bead (.5 um) first. I used more than four different beads, (4 different sets one for each); last set contains more than 1 bead. I am discussing parameters, process and the results.		I have done power spectrum analysis for small bead (.5 um) first. I used more than four different beads, (4 different sets one for each); last set contains more than 1 bead. I am discussing parameters, process and the results.

Pranav Rathi: /* Parameters */

Pranav Rathi — Fri, 11 Feb 2011 23:26:58 GMT

Parameters

←Older revision		Revision as of 23:26, 11 February 2011
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	<div align="center"><html><div style="width:425px" id="__ss_5746051"><strong style="display:block;margin:12px 0 4px"><a href="http://www.slideshare.net/pranavrathi/smallbeadpwsp2" title="Smallbeadpwsp2">Smallbeadpwsp2</a></strong><object id="__sse5746051" width="425" height="355"><param name="movie" value="http://static.slidesharecdn.com/swf/ssplayer2.swf?doc=smallbeadpwsp2-101111154335-phpapp02&stripped_title=smallbeadpwsp2&userName=pranavrathi" /><param name="allowFullScreen" value="true"/><param name="allowScriptAccess" value="always"/><embed name="__sse5746051" src="http://static.slidesharecdn.com/swf/ssplayer2.swf?doc=smallbeadpwsp2-101111154335-phpapp02&stripped_title=smallbeadpwsp2&userName=pranavrathi" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="425" height="355"></embed></object><div style="padding:5px 0 12px">View more <a href="http://www.slideshare.net/">presentations</a> from <a href="http://www.slideshare.net/pranavrathi">pranavrathi</a>.</div></div></html></div>		<div align="center"><html><div style="width:425px" id="__ss_5746051"><strong style="display:block;margin:12px 0 4px"><a href="http://www.slideshare.net/pranavrathi/smallbeadpwsp2" title="Smallbeadpwsp2">Smallbeadpwsp2</a></strong><object id="__sse5746051" width="425" height="355"><param name="movie" value="http://static.slidesharecdn.com/swf/ssplayer2.swf?doc=smallbeadpwsp2-101111154335-phpapp02&stripped_title=smallbeadpwsp2&userName=pranavrathi" /><param name="allowFullScreen" value="true"/><param name="allowScriptAccess" value="always"/><embed name="__sse5746051" src="http://static.slidesharecdn.com/swf/ssplayer2.swf?doc=smallbeadpwsp2-101111154335-phpapp02&stripped_title=smallbeadpwsp2&userName=pranavrathi" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="425" height="355"></embed></object><div style="padding:5px 0 12px">View more <a href="http://www.slideshare.net/">presentations</a> from <a href="http://www.slideshare.net/pranavrathi">pranavrathi</a>.</div></div></html></div>
		+
		+	<div align="center"><html><div style="width:425px" id="__ss_6897823"><strong style="display:block;margin:12px 0 4px"><a href="http://www.slideshare.net/pranavrathi/assignment-02152011" title="Assignment 02152011">Assignment 02152011</a></strong><object id="__sse6897823" width="425" height="355"><param name="movie" value="http://static.slidesharecdn.com/swf/ssplayer2.swf?doc=assignment02152011-110211171612-phpapp02&stripped_title=assignment-02152011&userName=pranavrathi" /><param name="allowFullScreen" value="true"/><param name="allowScriptAccess" value="always"/><embed name="__sse6897823" src="http://static.slidesharecdn.com/swf/ssplayer2.swf?doc=assignment02152011-110211171612-phpapp02&stripped_title=assignment-02152011&userName=pranavrathi" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="425" height="355"></embed></object><div style="padding:5px 0 12px">View more <a href="http://www.slideshare.net/">presentations</a> from <a href="http://www.slideshare.net/pranavrathi">pranavrathi</a>.</div></div></html></div>

	===Process & Results===		===Process & Results===

Pranav Rathi: /* Conclusion */

Pranav Rathi — Fri, 12 Nov 2010 20:43:01 GMT

Conclusion

←Older revision		Revision as of 20:43, 12 November 2010
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	===Conclusion===		===Conclusion===
	{{SJK Comment\|l=00:28, 12 November 2010 (EST)\|c=This page rocks!!! I agree I think we can unzip. Here are some comments from me: (1) the 1100bp-374 nm does not get converted to force. Rather how far we can go in the trap does. And actually, once you know stiffness you can calculate the max force by looking at the max deflection in the DOG signal--can explain better tomorrow. (2) I like this water objective! It's not actually that the stiffness is lower near the surface. With the water immersion, I think the stiffness stays the same, since aberrations are the same. Rather, the viscous drag force increases near the surface -- you can see this correction formula in the Svoboda and Block paper that you have. OK, Great work on this!}}		{{SJK Comment\|l=00:28, 12 November 2010 (EST)\|c=This page rocks!!! I agree I think we can unzip. Here are some comments from me: (1) the 1100bp-374 nm does not get converted to force. Rather how far we can go in the trap does. And actually, once you know stiffness you can calculate the max force by looking at the max deflection in the DOG signal--can explain better tomorrow. (2) I like this water objective! It's not actually that the stiffness is lower near the surface. With the water immersion, I think the stiffness stays the same, since aberrations are the same. Rather, the viscous drag force increases near the surface -- you can see this correction formula in the Svoboda and Block paper that you have. OK, Great work on this!}}
-	The stiffness is certainly better above 1 um from the surface which was expected. The overall stiffness is somewhat low at 1 W. The maximum power we can get before the objective is 1.4 W, which will give us 21pN/100nm. This is enough to ~~unzip~~ the ~~desired DNA (1100bp—374nm---77pN of~~ force). ~~I am happy that we are good to unzip (\| ;->)~~	+	The stiffness is certainly better above 1 um from the surface which was expected. The overall stiffness is somewhat low at 1 W. The maximum power we can get before the objective is 1.4 W, which will give us 21pN/100nm. This information is enough to estimate the force.

	==DOG==		==DOG==

Steven J. Koch: /* Conclusion */

Steven J. Koch — Fri, 12 Nov 2010 05:28:14 GMT

Conclusion

←Older revision		Revision as of 05:28, 12 November 2010
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	===Conclusion===		===Conclusion===
		+	{{SJK Comment\|l=00:28, 12 November 2010 (EST)\|c=This page rocks!!! I agree I think we can unzip. Here are some comments from me: (1) the 1100bp-374 nm does not get converted to force. Rather how far we can go in the trap does. And actually, once you know stiffness you can calculate the max force by looking at the max deflection in the DOG signal--can explain better tomorrow. (2) I like this water objective! It's not actually that the stiffness is lower near the surface. With the water immersion, I think the stiffness stays the same, since aberrations are the same. Rather, the viscous drag force increases near the surface -- you can see this correction formula in the Svoboda and Block paper that you have. OK, Great work on this!}}
	The stiffness is certainly better above 1 um from the surface which was expected. The overall stiffness is somewhat low at 1 W. The maximum power we can get before the objective is 1.4 W, which will give us 21pN/100nm. This is enough to unzip the desired DNA (1100bp—374nm---77pN of force). I am happy that we are good to unzip (\| ;->)		The stiffness is certainly better above 1 um from the surface which was expected. The overall stiffness is somewhat low at 1 W. The maximum power we can get before the objective is 1.4 W, which will give us 21pN/100nm. This is enough to unzip the desired DNA (1100bp—374nm---77pN of force). I am happy that we are good to unzip (\| ;->)

Pranav Rathi: /* Conclusion */

Pranav Rathi — Thu, 11 Nov 2010 23:43:05 GMT

Conclusion

←Older revision		Revision as of 23:43, 11 November 2010
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	The stiffness is certainly better above 1 um from the surface which was expected. The overall stiffness is somewhat low at 1 W. The maximum power we can get before the objective is 1.4 W, which will give us 21pN/100nm. This is enough to unzip the desired DNA (1100bp—374nm---77pN of force). I am happy that we are good to unzip (\| ;->)		The stiffness is certainly better above 1 um from the surface which was expected. The overall stiffness is somewhat low at 1 W. The maximum power we can get before the objective is 1.4 W, which will give us 21pN/100nm. This is enough to unzip the desired DNA (1100bp—374nm---77pN of force). I am happy that we are good to unzip (\| ;->)

-		+	==DOG==



	[[Category:Optical Tweezer]]		[[Category:Optical Tweezer]]

Pranav Rathi: /* Set4-bead4 */

Pranav Rathi — Thu, 11 Nov 2010 23:41:56 GMT

Set4-bead4

←Older revision		Revision as of 23:41, 11 November 2010
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	Average stiffness is 15.35pN, and it is better above 1 um and it somewhat remains same from 1 to 19 um. The straight horizontal line on the plot is average stiffness over 25 um range.		Average stiffness is 15.35pN, and it is better above 1 um and it somewhat remains same from 1 to 19 um. The straight horizontal line on the plot is average stiffness over 25 um range.

-	[[Image:Stiffness vs z-piezo.jpg\|~~thumb\|800 × 600 pixels\|~~]]	+	[[Image:Stiffness vs z-piezo.jpg\|800x600px]]
-		+


		+	===Conclusion===
		+	The stiffness is certainly better above 1 um from the surface which was expected. The overall stiffness is somewhat low at 1 W. The maximum power we can get before the objective is 1.4 W, which will give us 21pN/100nm. This is enough to unzip the desired DNA (1100bp—374nm---77pN of force). I am happy that we are good to unzip (\| ;->)

Pranav Rathi: /* Set4-bead4 */

Pranav Rathi — Thu, 11 Nov 2010 23:28:12 GMT

Set4-bead4

←Older revision		Revision as of 23:28, 11 November 2010
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	====Set4-bead4====		====Set4-bead4====
	Started at the surface (z=0) and went up in 1um increments (At z-piezo .1um belongs to 20mVolts/ 25 um belongs to 5 volts) up to 25 um into the sample.		Started at the surface (z=0) and went up in 1um increments (At z-piezo .1um belongs to 20mVolts/ 25 um belongs to 5 volts) up to 25 um into the sample.
		+	Average stiffness is 15.35pN, and it is better above 1 um and it somewhat remains same from 1 to 19 um. The straight horizontal line on the plot is average stiffness over 25 um range.

	[[Image:Stiffness vs z-piezo.jpg\|thumb\|800 × 600 pixels\|]]		[[Image:Stiffness vs z-piezo.jpg\|thumb\|800 × 600 pixels\|]]

Pranav Rathi: /* Set3-bead3 */

Pranav Rathi — Thu, 11 Nov 2010 23:25:09 GMT

Set3-bead3

←Older revision		Revision as of 23:25, 11 November 2010
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	====Set3-bead3====		====Set3-bead3====
	Started at the surface (z=0) and went up in .5um increments (At z-piezo .1um belongs to 20mVolts) up to 15 um into the sample. Last three steps are of 5 um each.		Started at the surface (z=0) and went up in .5um increments (At z-piezo .1um belongs to 20mVolts) up to 15 um into the sample. Last three steps are of 5 um each.
		+	Average stiffness is 12.54pN, and it is better above 1 um.
		+
	====Set4-bead4====		====Set4-bead4====
	Started at the surface (z=0) and went up in 1um increments (At z-piezo .1um belongs to 20mVolts/ 25 um belongs to 5 volts) up to 25 um into the sample.		Started at the surface (z=0) and went up in 1um increments (At z-piezo .1um belongs to 20mVolts/ 25 um belongs to 5 volts) up to 25 um into the sample.