User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/29

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(Autocreate 2014/01/29 Entry for User:Puja_Mody/Notebook/AU_Biomaterials_572)
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==Entry title==
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==Day 2==
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* Insert content here...
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*Goals
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# Prepare a new BSA-Magnetite solution without any gold in order to synthesize magnetite nanoparticles which have been incorporated into the BSA fibers.  
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#Analyze the AuNP-BSA and Magnetite-AuNP-BSA solutions which were prepared on [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/28|01/28/2014]] using UV-Vis spectroscopy
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==Procedure==
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- We adopted a different method of preparation on this day in order to compare the effectiveness between the technique of today and [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/28| yesterday]]. This protocol was adopted from the following reference [[http://www.sciencedirect.com/science/article/pii/S002197970901488X]].
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*Preparation of Magnetite-BSA solution.
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#The Following stock solutions were prepared using deionized water.
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## A 1M stock solution of KOH in a 10mL volumetric flask
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## A 0.8M stock solution of KNO<sub>3</sub> in a 50mL volumetric flask.
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## A 0.3M stock solution of FeSO<sub>4</sub>*7H<sub>2</sub>O in a 10mL volumetric flask. 
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#Add the appropriate volume of BSA and gold in a 10mL test tube to obtain the desired ratio of BSA:Au. Adjust levels so that the total volume of the solution does not exceed 5mL at this time. 
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#Add the appropriate volume of FeSO<sub>4</sub>*7H<sub>2</sub>O to each test tube so that the solution has a concentration of 0.03M FeSO<sub>4</sub>*7H<sub>2</sub>O.
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#Add the appropriate volume of KOH to each test tube so that the solution has a concentration of 0.1M KOH.
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#Add the appropriate volume of KNO<sub>3</sub> to each test tube so that the solution has a concentration of 0.4M KNO<sub>3</sub>.
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#Add the necessary amount of water into the flask in order to bring the solution up to 5mL in volume. Seal the round bottom flask with a septum and make sure that it is secure. 
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# Use a Schlenk Line apparatus to funnel nitrogen gas into the solution thereby de-gassing it. Conduct this process at room temperatue for 5 minutes prior to heating the magnetite-BSA solution in a hot water bath at  90°C for 4 hours. Maintain a continuous flow of nitrogen gas throughout the mixture during this time.
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<br>
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*Note: A previously prepared stock solution of 15mM BSA was used for the preparation.
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Revision as of 22:38, 3 February 2014

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Day 2

  • Goals
  1. Prepare a new BSA-Magnetite solution without any gold in order to synthesize magnetite nanoparticles which have been incorporated into the BSA fibers.
  2. Analyze the AuNP-BSA and Magnetite-AuNP-BSA solutions which were prepared on 01/28/2014 using UV-Vis spectroscopy

Procedure

- We adopted a different method of preparation on this day in order to compare the effectiveness between the technique of today and yesterday. This protocol was adopted from the following reference [[1]].

  • Preparation of Magnetite-BSA solution.
  1. The Following stock solutions were prepared using deionized water.
    1. A 1M stock solution of KOH in a 10mL volumetric flask
    2. A 0.8M stock solution of KNO3 in a 50mL volumetric flask.
    3. A 0.3M stock solution of FeSO4*7H2O in a 10mL volumetric flask.
  2. Add the appropriate volume of BSA and gold in a 10mL test tube to obtain the desired ratio of BSA:Au. Adjust levels so that the total volume of the solution does not exceed 5mL at this time.
  3. Add the appropriate volume of FeSO4*7H2O to each test tube so that the solution has a concentration of 0.03M FeSO4*7H2O.
  4. Add the appropriate volume of KOH to each test tube so that the solution has a concentration of 0.1M KOH.
  5. Add the appropriate volume of KNO3 to each test tube so that the solution has a concentration of 0.4M KNO3.
  6. Add the necessary amount of water into the flask in order to bring the solution up to 5mL in volume. Seal the round bottom flask with a septum and make sure that it is secure.
  7. Use a Schlenk Line apparatus to funnel nitrogen gas into the solution thereby de-gassing it. Conduct this process at room temperatue for 5 minutes prior to heating the magnetite-BSA solution in a hot water bath at 90°C for 4 hours. Maintain a continuous flow of nitrogen gas throughout the mixture during this time.


  • Note: A previously prepared stock solution of 15mM BSA was used for the preparation.







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