User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/05

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Entry title)
(Day 4)
Line 8: Line 8:
==Day 4==
==Day 4==
* GOALS:
* GOALS:
-
#Run AAS on the BSA- Magnetite-Gold solutions prepared on 1/28.
+
#Prepare BSA with magnetite using the general protocol described on 1/28 and 1/29 for comparison. (oven and nitrogen)  
-
#Prepare Hemoglobin/ BSA with magnetite using the general protocol described on 1/28 and 1/29 for comparison. (oven and nitrogen)  
+
-
#At differing ratios of protein in order to make fibers.
+
#UV-Vis of samples prepared on 1/29 and 2/4 (Magnetite-BSA made under nitrogen and Magnetite-BSA in oven and Hemoglobin+magnetite made in oven and under nitrogen.
#UV-Vis of samples prepared on 1/29 and 2/4 (Magnetite-BSA made under nitrogen and Magnetite-BSA in oven and Hemoglobin+magnetite made in oven and under nitrogen.
-
#6 samples total
+
#UV Vis protocol described on 1/29 in [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/29|Moira’s Open wet ware]]
-
#UV Vis protocol described on 1/29 in Moira’s Open wet ware.   
+
#Prepare all the magnetite samples from last week for FTIR and X-ray analysis.   
 +
 
 +
==Procedure==
 +
'''Preparation of BSA-Magnetitie nanoparticles'''
 +
* We followed our general procedure from [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/01/29|1/29]].
 +
* We prepared two different solutions, one with the correct KNO<sub>3</sub> concentration and one with the incorrect one. For details on incorrect and correct concentrations see [[User:Puja Mody/Notebook/AU Biomaterials 572/2014/02/04|2/4]]
 +
 
 +
 
 +
 
 +
 
 +
 
 +
Table 1. Preparation of both reaction mixtures
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Ratio'''
 +
| align="center" style="background:#f0f0f0;"|'''concentration BSA (M)'''
 +
| align="center" style="background:#f0f0f0;"|'''volume of BSA added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''volume water added (mL)'''
 +
|-
 +
| 13000||2.30769E-06||0.459210041||0.5||0.500624554||2.5||1.040165405
 +
|}
 +
<br>
 +
Table 2. Preparation of BSA stock solution
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Preparation of BSA stock'''
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
|-
 +
| Molecular weight (g/mol)||66463
 +
|-
 +
| mass (g)||0.0167
 +
|-
 +
| volume stock (L)||0.01
 +
|-
 +
| Concentration (M)||2.51268E-05
 +
|}
 +
<br>
 +
Table 3. Preparation of FeSO<sub>4</sub>*7H<sub>2</sub>O stock solution
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Preparation of FeSO4 stock'''
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
|-
 +
| Molecular Weight (g/mol)||278.01
 +
|-
 +
| mass(g)||0.83403
 +
|-
 +
| volume stock (L)||0.01
 +
|-
 +
| Concentration (M)||0.3
 +
|}
 +
<br>
 +
Table 4. Preparation of KOH stock solution
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Preparation of KOH stock'''
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
|-
 +
| Molecular Weight (g/mol)||56.11
 +
|-
 +
| mass(g)||0.5604
 +
|-
 +
| volume stock (L)||0.01
 +
|-
 +
| Concentration (M)||0.998752451
 +
|}
 +
<br>
 +
 
 +
Table 5. Preparation of KNO<sub>3</sub> stock solution for Reaction Mixture 1
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
|-
 +
| Molecular Weight (g/mol)||101.1
 +
|-
 +
| mass(g)||4.044
 +
|-
 +
| volume stock (L)||0.05
 +
|-
 +
| Concentration (M)||0.8
 +
|}
 +
<br>
 +
Table 6. Preparation of KNO<sub>3</sub> stock solution for Reaction Mixture 2
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
|-
 +
| Molecular Weight (g/mol)||101.1
 +
|-
 +
| mass(g)||0.5604
 +
|-
 +
| volume stock (L)||0.01
 +
|-
 +
| Concentration (M)||0.5
 +
|}
 +
 
 +
 
 +
 
 +
 

Revision as of 10:00, 19 February 2014

Project name Main project page
Previous entry      Next entry

Day 4

  • GOALS:
  1. Prepare BSA with magnetite using the general protocol described on 1/28 and 1/29 for comparison. (oven and nitrogen)
  2. UV-Vis of samples prepared on 1/29 and 2/4 (Magnetite-BSA made under nitrogen and Magnetite-BSA in oven and Hemoglobin+magnetite made in oven and under nitrogen.
  3. UV Vis protocol described on 1/29 in Moira’s Open wet ware
  4. Prepare all the magnetite samples from last week for FTIR and X-ray analysis.

Procedure

Preparation of BSA-Magnetitie nanoparticles

  • We followed our general procedure from 1/29.
  • We prepared two different solutions, one with the correct KNO3 concentration and one with the incorrect one. For details on incorrect and correct concentrations see 2/4



Table 1. Preparation of both reaction mixtures

Ratio concentration BSA (M) volume of BSA added (mL) amount of FeSO4 added (mL) amount of KOH added (mL) amount of KNO3 added (mL) volume water added (mL)
130002.30769E-060.4592100410.50.5006245542.51.040165405


Table 2. Preparation of BSA stock solution

Preparation of BSA stock '
Molecular weight (g/mol)66463
mass (g)0.0167
volume stock (L)0.01
Concentration (M)2.51268E-05


Table 3. Preparation of FeSO4*7H2O stock solution

Preparation of FeSO4 stock '
Molecular Weight (g/mol)278.01
mass(g)0.83403
volume stock (L)0.01
Concentration (M)0.3


Table 4. Preparation of KOH stock solution

Preparation of KOH stock '
Molecular Weight (g/mol)56.11
mass(g)0.5604
volume stock (L)0.01
Concentration (M)0.998752451


Table 5. Preparation of KNO3 stock solution for Reaction Mixture 1

Preparation of KNO3 '
Molecular Weight (g/mol)101.1
mass(g)4.044
volume stock (L)0.05
Concentration (M)0.8


Table 6. Preparation of KNO3 stock solution for Reaction Mixture 2

Preparation of KNO3 '
Molecular Weight (g/mol)101.1
mass(g)0.5604
volume stock (L)0.01
Concentration (M)0.5





Personal tools